DEX induces displacement of PI-PLC, Src, and GR from HSP90 but does not affect PI-PLC/Src association.
Cellular extracts from murine thymocytes, untreated or treated with DEX (10−7 M) for the indicated times (5 or 30 minutes), were immunoprecipitated (IP) with anti-HSP90 (A) or anti-Src antibodies (B). Western blot analyses were performed with the indicated antibodies. (C) Murine thymocytes were treated with PP1 (10 μM), U73122 (2.5 μM), and D609 (50 μg/mL) 30 minutes before DEX treatment (10−7 M, 5 minutes). Tyrosine-phosphorylated proteins were immunoprecipitated with agarose-conjugated 4G-10 antibodies, and PI-PLC abundance in the 4G-10 immunoprecipitates was assessed by Western blot with anti–PI-PLC monoclonal antibody (C, bottom). Whole lysates were run to control the PI-PLC levels (C, top).
