DEX and C2 ceramide induce caspase-8–FADD association.

(A) Thymocytes, untreated or treated for the indicated times (0.5-3 hours) with DEX (10⁻⁷ M) or C2 ceramide (50 μM), were immunoprecipitated with anti-FADD monoclonal antibody and immunoprecipitates revealed with anti–caspase-8 and anti-FADD antibodies. (B) Thymocytes, untreated or treated for different times (0.5-3 hours) with DEX (10⁻⁷ M) or C2 ceramide (50 μM), were immunoprecipitated with anti–caspase-8 antibody and immunoprecipitates revealed with anti-FADD and anticaspse-8 antibodies. (C) Thymocytes, untreated or treated for different times (1-6 hours) with C2 ceramide (50 μM), were assayed for caspase-8 cleavage by Western blot with anti–caspase-8 antibody.