Coomassie and immunostaining of proteins from band 3(−/−) mouse RBC membranes.

RBC membranes were separated on 10% or 12% Laemmli gels and immunoblotted using polyclonal antibodies as shown. Loading: C1, C2, controls 1 and 2, respectively; M1, M2, band 3(−/−) mice 1 and 2, respectively (C1 was C57BL/6 strain; M1, M2, and C2 were B6.129 strain). H and L on the protein-stained gel indicate the immunoglobulin H and L chains in the band 3(−/−) membranes. C-term indicates C-terminal.