Retroviral vector and time course for administration of HGF and RV to adult mice.

(A) Retroviral vector hAAT-cFIX–WPRE. The MLV-based RV contains intact long terminal repeats (LTRs) at the 5′ and 3′ ends, an extended packaging signal (ψ⁺), the 403-nt human α₁-antitrypsin promoter (hAAT), the 1.5-kb cFIX, and the 591-nt woodchuck hepatitis posttranscriptional regulatory element (WPRE). Transcription can initiate from the LTR or the hAAT promoters as indicated by the arrows. (B) Time course for administration of HGF and RV to adult BALB/c mice. Six-week-old BALB/c mice were injected intraperitoneally with 5 doses of HGF every 3 hours between 0 and 12 hours (arrows) for a cumulative dose of 29 mg/kg. Controls received PBS at the same times. Equal doses of RV were then injected intravenously into both groups at 30, 36, and 48 hours (arrowheads) after the first dose of HGF or PBS, for a cumulative dose of 1 × 1010 TU/kg.