Assessment of colocalization with induced clusters.

The coincident intensity index is the area of coincident intensity (shown in yellow in Figure 2) normalized by the area of noncoincident intensities (red + green). The average ± standard deviation for at least 15 cells is plotted for each inducer. The maximum apparent range of this index is established from double labeling of CD47 with the noncompeting antibody 6H9 and cluster-inducing PhE-BRIC-126. The minimum apparent range of this index is established from labeling CD47 with PhE-BRIC-126 together with the lipid bilayer. Cytoskeletal F-actin, Band 3, and glycophorin C do not appear to be colocalized with CD47. In comparison, both RhAG and RhcE appear to colocalize in these clusters with CD47. Additionally, RhAG is colocalized to clusters induced by polyclonal anti-Rh(c and E).