Dose-dependent inhibition of PMA-elicited oxidative burst of human monocytes by selected OH-PUFA.

Adherent monocytes were incubated for 10 minutes with 12-HETE (■), 15-HETE (▨), arachidonic acid (▪), 13-HODE (▥), and 13-HpODE (dotted bars, close to nil) in the humidified CO₂/air incubator at 37°C. After changing medium to remove additions, PMA-elicited oxidative burst was quantified at burst peak by measuring luminescence in a luminol-dependent assay. Values represent the percentages of inhibition of PMA-elicited oxidative burst in treated versus untreated control monocytes. For details, see “Materials and methods.” Data are expressed as means ± SD of 3 separate experiments.