Cytotoxic activity of LMP1-specific CTL lines.

LMP1-specific CTL lines were tested for their ability to lyse autologous targets. (A) Autologous PHA blasts of donor A and C loaded with YLQ peptide were killed in cytotoxicity assays by LMP1-specific CTLs at an effector-target ratio of 40:1. PHA blasts alone or loaded with a control peptide (YLL) were not lysed. (B) Autologous fibroblasts of donor B expressing LMP1 and ΔLMP1 were killed by LMP1-specific CTLs. Nontransduced fibroblasts and fibroblasts transduced with an adenoviral vector without transgene were not lysed. (C) LMP1-specific CTLs of donor A killed autologous LCLs. No cytotoxic activity was observed against mismatched targets.