Cytotoxic activity profile of LMP1- and EBV-specific CTLs.

(A) LMP1-specific CTLs were generated by DC_ΔLMP1 stimulations followed by autologous LCL stimulations and (B) EBV-specific CTLs by LCL stimulations alone. Both were tested at an effector-target ratio of 40:1 against a panel of autologous targets, including autologous LCLs (LCL), mismatched LCLs (MM), fibroblasts alone (−), fibroblasts infected with recombinant vaccinia virus expressing EGFP, EBNA-3A, -3B, or -3C, or fibroblasts infected with recombinant adenovirus without transgene (Ad) or ΔLMP1. Both CTL lines killed autologous LCLs; however, only LMP1-specific CTLs killed LMP1-expressing targets. No cytotoxic activity of LMP1-specific CTLs was observed against EBNA-3B– and -3C–expressing targets in contrast to EBV-specific CTLs.