MAGE-3 sequence segments recognized by CD4⁺T cells from 6 donors and their HLA-DR type.

Polyclonal CD4⁺ T-cell lines propagated in culture with pool I and pool II were tested with each single peptides (10 μg/mL) forming the pools in 2-day microproliferation assays. The results are representative of several assays in the case of donors 1, 2, 4, and donor/patient 5 and, at least 2 experiments, in the case of donor 3 and donor/patient 6. Peptides that elicited a strong response are indicated in black (P < .001), peptides that elicited an intermediate response are indicated in hatched segments (.001 < P < .05), and peptides that elicited a low but still significant response are indicated in white (P < .05). Responses significantly higher than the blanks (ie, the basal level of proliferation of CD4⁺ T cells in the presence of autologous PBMCs as APCs) were determined by unpaired, one-tailed Student t test. NT indicates not tested.