Subpopulations of peripheral blood CLA+ (and other) memory TH cells express different levels of CCR10.
(A) CLA+ “cutaneous” TH cells can be further divided into distinct subpopulations based on CD27 and CCR7 expression. Top: CD27 versus CCR7 expression on CLA+ memory TH cells. Bottom: CLA versus CD27 (left plot) or CLA versus CCR7 (right plot) for a representative healthy donor. (B) Bar graph showing proportion of CLA+ memory TH cells expressing the 4 possible permutations of CD27 and CCR7 expression. Mean and SD of 10 healthy donors shown. (C) Cytometry plots of CCR10 or CCR4 expression by adhesion receptor–defined memory subsets further divided by CD27 and CCR7 expression on representative donor blood. CCR10 staining (black lines) overlaid on isotype-matched control staining (gray lines). Naive, CLA+, and CLA−plots are all from the same 6-color stain. The β7+ and β7− populations are from a separate 6-color stain of the same donor. (D) Statistical analysis of CCR10 and CCR4 expression on 10 healthy donors by subsets gated as in B. The difference in CCR10 expression between each of the 2 CLA+ populations and all other populations is significant (P < .05). There is no significant difference in CCR4 expression between the 2 CLA+ populations.
