Angiostatin inhibits HGF-induced phosphorylation of Akt in HUVECs.

HUVECs in serum-free medium were unstimulated (lane 1) or treated with HGF (10 ng/mL) in the absence (lane 2) or presence (lanes 3-7) of angiostatin. Angiostatin concentrations were 0.5 μM (lane 3), 0.75 μM (lane 4), 1.0 μM (lane 5), 2 μM (lane 6), or 3 μM (lane 7). After a 5-minute incubation, cell lysates were prepared and immunoblotting for phospho-AKT was performed as described. The blot was stripped and re-probed for total Akt.