Angiostatin does not inhibit VEGF- or bFGF-induced phosphorylation of Akt.

HUVECs (A) or HASMCs (B) in serum-free medium were unstimulated (lane 1) or treated with VEGF (50 ng/mL) in the absence (lane 2) or presence (lane 3) of angiostatin (ANG; 5 μM). Similarly, treatment with bFGF, 25 ng/mL (lane 4) resulted in AKT phosphorylation that was not inhibited by angiostatin, 5 μM (lane 5).