Plasminogen does not inhibit HGF-induced phosphorylation of Akt.

HUVECs in serum-free medium were unstimulated or treated with HGF for 5 minutes with or without plasminogen at varying concentrations. Lane 1 is control (unstimulated cells); lane 2, HGF (10 ng/mL); lanes 3-5, HGF (10 ng/mL) plus plasminogen, at 0.5μM (lane 3), 1.0 μM (lane 4), and 3 μM (lane 5); lane 6, plasminogen at 3 μM without HGF; lane 7, HGF (10 ng/mL) and angiostatin (3 μM). Cell lysates were prepared for immunoblotting to detect phospho-AKT, then total AKT (after stripping).