Angiostatin selectively inhibits HGF-induced proliferation of HUVECs.

HUVECs (5000 cells/well) were cultured in 96-well plates in serum in the presence or absence of HGF (10 ng/mL) or bFGF (10 ng/mL). Angiostatin was added to some wells at 3 μM. After 72 hours of culture, a metabolic cell proliferation assay was performed with monitoring of formazan production at 490 nm. Each assay was performed in replicates of 6. Angiostatin significantly (P < .01) inhibited HGF-induced proliferation, but did not inhibit bFGF-induced proliferation. Angiostatin also failed to significantly reduce VEGF-induced proliferation (data not shown). Error bars indicate ± 1 SD.