C/EBPα inhibits monocytic differentiation.

(A) The morphology of 32DPKCδ-αER-1 cells was assessed with no additions (−), after 24 hours in estradiol (E), 8 hours in PMA (P), or 8 hours in PMA and estradiol (P/E). The morphologies of the αER-2 and ER lines in PMA or PMA + estradiol for 8 hours are also shown. (B-D) Total cellular RNAs were prepared from the indicated cell lines cultured in PMA, estradiol (E2), both, or neither for the indicated number of hours. These RNAs, 10 μg per lane, were then subjected to Northern blotting for macrosialin (MS), myeloperoxidase (MPO), lactoferrin (LF), C/EBPε, PU.1, and β-actin. If no transcripts were detected, the results are not shown.