Inhibition of monocytic differentiation by C/EBPα depends upon its basic region.

(A) The morphology of 32DPKCδ-Leu12Val, -BR3, -Δ3-8, and -GZ cells exposed to PMA (P) or PMA + estradiol (P/E) for 8 hours is shown. (B-E) RNAs prepared from these cell lines cultured in PMA, estradiol (E2), both, or neither for the indicated times were subjected to Northern blotting sequentially for macrosialin (MS), myeloperoxidase (MPO), C/EBPε, PU.1, and β-actin. MPO RNA was not detected (not shown).