Fig. 6.
The C/EBPα basic region is required to inhibit JunB protein and RNA expression.
(A) Nuclear extracts prepared from the indicated 32DPKCδ cell lines 0, 0.5, 8, or 24 hours after addition of estradiol (E2) were subjected to gel-shift assay using the AP-1 oligonucleotide. A 0.5-hour GZ sample was not prepared. (B) Total cellular protein extracts from 1 × 106 Leu12Val, BR3, or GZ cells cultured for 8 hours in PMA, estradiol, both, or neither were subjected to Western blotting for JunB and β-actin. (C) Total cellular RNAs (10 μg) from αER-1, Leu12Val, or BR3 cells were subjected to Northern blotting for JunB and β-actin.
