Changes in MAP kinase phosphorylation patterns following apicidin treatment of K562 cells.

Cells were treated with 0.5 μM apicidin for the various times indicated and harvested, and 20 μg cell lysate was subjected to Western blot analysis by using specific antibodies against phosphorylated p38, ERK1/2, and JNK1/2, respectively. Blots were stripped and reprobed with pan antibodies against p38, ERK1/2, and JNK1/2, referred to as total in the figure. Lower panel shows HbF synthesis during the course of the experiment. Similar results were obtained in a second series of experiments.