Fig. 7.
![Fig. 7. Activation of Aγ-globin gene promoter by apicidin in K562 cells. / Cells were transiently transfected with a 1435-bp Aγ-promoter/luciferase reporter gene construct and subsequently treated with 0.5 μM apicidin for the various times indicated. After determination of luciferase activity from cell lysates as described in “Materials and methods,” values were expressed relative to the activity of the 0 [h] value. Hatched bars show results of transfected cells that have been treated with p38 inhibitor SB203580 prior to addition of apicidin. Each experiment was performed 3 times, and standard errors were calculated as indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/5/10.1182_blood-2002-08-2617/3/h80533885007.jpeg?Expires=1735833502&Signature=eyh6gJODMaylwabIFmnuY2wnhm7CPIMXU89FLvjVu4oejcdoMMjwAWjo87m~vGlywh1IsfF4RPdWPFTRB2~wYDsleECzixJOkoJwj0QJi1uiSIOzF0IiTeiCJAXeQYzXU4JGmdprbmkB0XZHKPSUlEUroYkbb-Ge0zWY3vas3DSSrzAk5ExKoIeW3PdinLFdyRVTzYSlHWy1bJUXFELHQtHNr6pV3lRsTJeONr7XO5l15WodpbAqPDYxZoAo7b0GSQfQvksBlDMRi2m~lyx-9sMsYGtkBhhoX0tbBwn7oQjY8fxRtQ0FAWvs80eoGnnYqqRF96wwLDFNTWe6ytM~pw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of Aγ-globin gene promoter by apicidin in K562 cells.
Cells were transiently transfected with a 1435-bp Aγ-promoter/luciferase reporter gene construct and subsequently treated with 0.5 μM apicidin for the various times indicated. After determination of luciferase activity from cell lysates as described in “Materials and methods,” values were expressed relative to the activity of the 0 [h] value. Hatched bars show results of transfected cells that have been treated with p38 inhibitor SB203580 prior to addition of apicidin. Each experiment was performed 3 times, and standard errors were calculated as indicated.
