Effect of the magnitude of NPP-mediated permeabilization and of hemoglobin consumption on the predicted relative cell volume changes of parasitized red cells.

In both panels, the curves predicted using the default parameter values for NPP_K-max and H_max (curves labeled A) are compared with those obtained using the slightly different values indicated for the curves labeled B. To incorporate the findings of Staines et al,15 all simulations were performed with approximately 50% Na-pump inhibition after 35 hours. Comparison of Figure 2C with curve A, top panel of Figure 4, shows that the predicted volume changes with or without Na-pump inhibition were very similar. Note that the default values of NPP_K-max and H_max, based on the measured values of these parameters, are near the critical limits (shown as horizontal lines) required to preserve host cell integrity to the end of the asexual cycle.