Transplantation of human CD34⁺ cells into NOD/SCID/MPSVII mice led to engraftment of both lymphoid and myeloid lineages.

Bone marrow from a NOD/SCID/MPSVII mouse 12 weeks after the transplantation of 5 × 10⁵ CD34⁺ cells from human cord blood was analyzed by flow cytometry. (A) A gate (G1) was drawn to exclude most of the positive cells in a sample stained with an isotype control for an antihuman CD45 antibody. (B) Staining for human CD45 showed 86.7% of bone marrow cells to fall within G1. Such positive cells were gated to the lower set of plots for analysis with additional lineage markers and isotype controls. (C) The specificity of the antibody used against human CD45 was demonstrated by a lack of staining of bone marrow from a NOD/SCID/MPSVII mouse that did not receive a transplant. (D) Human CD45⁺ cells gated from G1 exhibited minimal staining with isotype control antibodies for lineage markers. (E) A human CD19⁺ population but no human CD3⁺ cells were seen in G1-gated human CD45⁺cells. (F) Several populations expressing either human CD66b alone, human CD14 alone, or both markers together were evident in G1-gated human CD45⁺ cells.