Immunohistochemical staining for human CD45 colocalized with GUSB histochemical staining in the liver of a NOD/SCID/MPSVII mouse following transplantation of human CD34⁺ cells.

(A) A cell in the liver of a NOD/SCID/MPSVII mouse 12 weeks after the transplantation of human cord blood CD34⁺ cells shows black membrane staining following immunohistochemistry for human CD45. (B) After staining for both human CD45 and GUSB activity, a cell in the same NOD/SCID/MPSVII liver as in (A) shows both black membrane signal for human CD45 and red cytoplasmic signal for GUSB activity. GUSB enzyme activity was seen only in the presence of black membrane staining for human CD45. In addition, all human CD45⁺ cells exhibited GUSB staining. Original magnification, × 833.