American Society of Hematology

Fig. 1.

$Fig. 1. Resting endothelial cells bind but do not internalize125I–anti-ICAM. / (A) HUVECs were incubated with 125I–anti-ICAM or125I-IgG (1 hour, 37°C), and radioactivity was determined in the surface fraction (glycine elution, ▨) and in the cell lysates (▪). (B) Percent of internalization of125I-labeled antibodies against ACE, TM, and ICAM-1 by HUVECs at either 4°C (▪) or 37°C (▨). The data are expressed as means ± SD (n = 3).$

Resting endothelial cells bind but do not internalize¹²⁵I–anti-ICAM.

(A) HUVECs were incubated with ¹²⁵I–anti-ICAM or¹²⁵I-IgG (1 hour, 37°C), and radioactivity was determined in the surface fraction (glycine elution, ▨) and in the cell lysates (▪). (B) Percent of internalization of¹²⁵I-labeled antibodies against ACE, TM, and ICAM-1 by HUVECs at either 4°C (▪) or 37°C (▨). The data are expressed as means ± SD (n = 3).

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