Fig. 6.
Fig. 6. Localization of anti-ICAM and anti-ICAM/β-Gal in the pulmonary vasculature after injection in mice. / (A) Immunogold electron microscopy of the lungs harvested 4 hours after intravenous injection of 100 μg anti-ICAM (i) or control IgG (ii). Arrows show endothelium-associated gold particles. RBC indicates red blood cells in a capillary lumen; Lu, vascular lumen; Alv, alveolar compartment; EC, endothelial cell; EC-Nu, endothelial cell nucleus. (B) Targeting of an active β-Gal conjugate was visualized 1 hour after injection in mice, using standard X-Gal chromogenic substrate staining protocol. Distribution of anti-ICAM/β-Gal (panels i-iv) and control IgG/β-Gal (panels v-viii). Detailed view of anti-ICAM/β-Gal in the lungs (panels ix and x). Original magnifications: Ai, × 70 000; Aii, × 60 000; Bi-Bviii, × 10; Bix-Bx, × 20.

Localization of anti-ICAM and anti-ICAM/β-Gal in the pulmonary vasculature after injection in mice.

(A) Immunogold electron microscopy of the lungs harvested 4 hours after intravenous injection of 100 μg anti-ICAM (i) or control IgG (ii). Arrows show endothelium-associated gold particles. RBC indicates red blood cells in a capillary lumen; Lu, vascular lumen; Alv, alveolar compartment; EC, endothelial cell; EC-Nu, endothelial cell nucleus. (B) Targeting of an active β-Gal conjugate was visualized 1 hour after injection in mice, using standard X-Gal chromogenic substrate staining protocol. Distribution of anti-ICAM/β-Gal (panels i-iv) and control IgG/β-Gal (panels v-viii). Detailed view of anti-ICAM/β-Gal in the lungs (panels ix and x). Original magnifications: Ai, × 70 000; Aii, × 60 000; Bi-Bviii, × 10; Bix-Bx, × 20.

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