Inhibition of IL-1–induced human CRP promoter activity by PPAR-α activators in HuH7 cells.

HuH7 cells were transiently transfected with the human CRP promoter (pCRP-luc) linked to a luciferase reporter and incubated with increasing concentrations (10, 30, 50, 125, 250 μM) of gemfibrozil (GF), ciprofibrate (CF), bezafibrate (BF), and Wy 14643 (WY) or BRL49653 (BRL) (10 μM) for 5 hours and subsequently stimulated with IL-1 (10 ng/mL) for 18 hours. Luciferase activities are expressed as means ± SDs of several (3 or more) transfection experiments performed in triplicate.