Effect of PPAR-α activators on the IL-1–induced formation of C/EBP-β–p50-NFκB complexes.

Coimmunoprecipitation was performed on nuclear extracts prepared from HuH7 cells (A) and freshly isolated primary human hepatocytes (B), preincubated for 6 hours with Wy 14643 (WY), fenofibric acid (FFA), or vehicle, and subsequently stimulated with 10 ng/mL IL-1 (HuH7 cells) or 25 ng/mL IL-1 (primary hepatocytes) for 17 hours. PPAR-α activators were used at a final concentration of 50 μM and 250 μM in HuH7 cells and primary hepatocytes, respectively. Anti–p50-NFκB or anti–MMP-8 control antibody (Con) were used for immunoprecipitation. C/EBP-β bound to precipitated p50-NFκB was detected by Western blot analysis.