Effect of PPAR-α activators on the expression of C/EBP-β, p50-NFκB, and IκB-α in HuH7 cells.

(A) HuH7 cells were preincubated with Wy 14643 (WY; 50 μM), ciprofibrate (CF; 125 μM), or DMSO (Con) for 5 hours and subsequently were stimulated with 10 ng/mL IL-1 for 17 hours. Total cellular (upper panel) and nuclear (lower panel) extracts were analyzed for p50-NFκB and C/EBP-β expression. (B) HuH7 cells were incubated with PPAR-α activators for 24 hours, and total cellular extracts were analyzed for IκB-α by Western blotting. Equal loading was ensured by demonstration of uniform β-actin and histone H1 expression for total and nuclear extracts, respectively.