Fig. 1.
Fig. 1. Flow cytometric histograms of bcl-2 and bax expressions on 3 AML samples. / AML cases were analyzed by flow cytometry after exposure to anti-CD33 PE and anti–bcl-2 FITC or bax MoAbs, as described. (A) Bivariate histogram FSC/SSC with “blast gate” on leukemic cells and IgG1 isotype-negative controls for bcl-2 (B) or bax (C) and CD33 MoAbs from “blast gate.” Bcl-2 (Di, Ei, Fi) and bax (Gi, Hi, Ii) were evaluated on the CD33+ cells segregated by “AML cells” gate in 3 AML cases. Bcl-2 (Dii, Eii, Fii) or bax (Gii, Hii, Iii) univariate histograms, conditioned on “AML cells” gate, show the distribution of fluorescence, reported as arithmetic means (M).

Flow cytometric histograms of bcl-2 and bax expressions on 3 AML samples.

AML cases were analyzed by flow cytometry after exposure to anti-CD33 PE and anti–bcl-2 FITC or bax MoAbs, as described. (A) Bivariate histogram FSC/SSC with “blast gate” on leukemic cells and IgG1 isotype-negative controls for bcl-2 (B) or bax (C) and CD33 MoAbs from “blast gate.” Bcl-2 (Di, Ei, Fi) and bax (Gi, Hi, Ii) were evaluated on the CD33+ cells segregated by “AML cells” gate in 3 AML cases. Bcl-2 (Dii, Eii, Fii) or bax (Gii, Hii, Iii) univariate histograms, conditioned on “AML cells” gate, show the distribution of fluorescence, reported as arithmetic means (M).

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