Fig. 4.
p27KIP1 protein levels correlate with the induction of apoptosis.
(A) Day-6 DCs were harvested, extensively washed, and further cultured in RPMI-10% FCS in the presence or absence of IL-4 and GM-CSF (left panel) or in the presence of both IL-4 and GM-CSF with or without LY294002 (20 μM) or DMSO (right panel). Data demonstrated in left and right panels are derived from different experiments with different donors. (B) Freshly isolated peripheral blood monocytes were cultured in RPMI-10% FCS with or without addition of Rapa (1 μM).
