Fig. 2.
Effect of ATO on the cell cycle and apoptosis of RPMI 8226 cells.
(A) Cell cycle. (B) Apoptosis. RPMI 8226 cells expressing mutated p53 were cultured for 48 hours with 0 to 4 μM ATO. Cell cycle distribution was determined by the propidium iodide staining method, and stained cells were analyzed by flow cytometry using the ModFit software; 10 000 cells were analyzed. Apoptosis was determined by the annexin V method. For additional experimental details, see “Materials and methods.” Note the time- and dose-dependent arrest of cells in G2/M and the parallel kinetics of cell cycle arrest and extent of apoptosis. At least 3 experiments were performed, and 1 representative experiment is shown.
