Development of a model system for adhesion of thrombasthenic platelets to subendothelial structures under flow conditions and the effect of the thrombin-generating system.

Washed normal platelets and red cells (200 000 platelets/μL; hematocrit 40%) were perfused over PMA-ECM for 5 minutes at 1600 s⁻¹ in the absence (A) or presence (B-C) of an RGD-containing peptide (rRGDW; 200 μM). Panel C shows the effect of the thrombin-generating system (1.2 μg/mL rFVIIa, 10 μg factor X, 20 ng/mL prothrombin, 3 mM CaCl₂) on adhesion of RGD-inhibited platelets. Original magnification × 400 for panels A-C.