Binding of rFVIIa to collagen-adhered platelets under flow conditions.

Washed platelets and red cells were perfused over a collagen-coated glass coverslip in the absence (A) or presence (B) of rFVIIa (1.2 μg/mL). After perfusion, coverslips were fixed and bound rFVIIa was visualized by confocal laser scanning fluorescence microscopy using a monoclonal antibody against factor VII followed by a FITC-labeled secondary antibody. Original magnification × 400 for panels A-B.