PCR analysis of in vivo marking.

Representative PCR of neo and β-actin sequences in PB mononuclear cells (MNCs) and granulocytes (Gran) at different time points after engraftment in 3 animals. Concurrentβ-actin PCR was performed for each sample to quantitate the amount of amplifiable template DNA. Serial dilutions of G1Na DNA (2 copies of integrated vector per cell) into normal rhesus PB DNA were used as positive controls. In animals RQ2314 and RQ2277 the only amplified band corresponds to the vector that was used to transduce G-CSF+SCF–mobilized cells. In animal RQ2237 in addition to the band corresponding to G-CSF+SCF aliquot, a very faint band can be seen that corresponds to transduced G-CSF–mobilized cells.