Comparison of the ratio of in vivo gene marking versus the ratio of infused cells on a logarithmic scale.

In each animal the ratio of the average neo in vivo marking of first-year PB samples originating from G-CSF+SCF–mobilized cells to G-CSF alone or G-CSF+Flt3-L–mobilized cells were calculated (▪). For each animal this ratio was compared with the ratio of infused cells (ie, number of cells collected at the end of transduction) from G-CSF+SCF–mobilized cells to G-CSF alone or G-CSF+Flt3-L–mobilized cells (▨). Note that although the number of cells infused from the G-CSF+SCF aliquot was always higher in each animal compared with the other aliquot, the difference in gene marking levels between the aliquots was in every case significantly greater, except in animal RQ2265, which overall had low level marking. Monkeys RQ2314, RQ2284, and RQ2237 were mobilized with G-CSF+SCF versus G-CSF alone, and monkeys RQ2277 and RQ2265 were mobilized with G-CSF+SCF versus G-CSF + Flt3-L.