American Society of Hematology

Fig. 5.

$Fig. 5. Southern blot analysis of genomic marking. / In animals RQ2277 and RQ2314 the Southern blot analysis of PB mononuclear cells and granulocytes after engraftment shows only the vector that was used to transduce the G-CSF+SCF fraction (G1Na in animal RQ2277, and LNL6 in animal RQ2314). LNL6 DNA and serial dilutions of G1Na DNA into normal rhesus DNA were used as controls. Due to residual env sequences in LNL6 that are not present in G1Na, the expected fragment size is 3.0 kb for LNL6 and 2.3 kb for G1Na.$

Southern blot analysis of genomic marking.

In animals RQ2277 and RQ2314 the Southern blot analysis of PB mononuclear cells and granulocytes after engraftment shows only the vector that was used to transduce the G-CSF+SCF fraction (G1Na in animal RQ2277, and LNL6 in animal RQ2314). LNL6 DNA and serial dilutions of G1Na DNA into normal rhesus DNA were used as controls. Due to residual env sequences in LNL6 that are not present in G1Na, the expected fragment size is 3.0 kb for LNL6 and 2.3 kb for G1Na.

This Feature Is Available To Subscribers Only