Fig. 3.
![Fig. 3. BCR-ABL kinase activity in K562 and K562-R cells. / BCR-ABL was immunoprecipitated from cell lysates (400 μg) with anti-abl, washed extensively, resuspended in kinase buffer, and incubated with 500 nM STI571 or buffer alone (control) for 30 minutes. Kinase reactions were initiated by the addition of 10 μCi (0.37 MBq) [32P]-ATP and enolase. After 30 minutes at 25°C, reactions were quenched with the addition of 2 × sample buffer, and phosphoproteins were resolved by SDS-PAGE. Tyrosine phosphoproteins were detected by autoradiography. Autophosphorylation of BCR-ABL and phosphorylation of enolase are shown. Similar results were obtained using anti-bcr for immunoprecipitation (data not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/2/10.1182_blood.v101.2.690/4/h80233696003.jpeg?Expires=1736044224&Signature=revyZMMKz3ex0UXunmMZJqkZcGCOSj5xl3xlFVoCNSEsrNnDfDNKBVE3ZK9Z2swpXbPPKdaKDCd7jDs~wp0Nc7caOKMuNiHyE577ZrkZjCNwpPTTfBjO~ODhP6xRo-tF3KKzjZcEzPctWA5WwYrhAG~Awv1vOM-YlZknvDQf6HTi~NavMoYWRvV~O-PXCT1lF~2LFmebsXRoSfU9~aYOg-9wmNHH1TCHyifx-cLziD-pwlyXYA~WHN78g6QxpViXgqU8WyLJyIAMnL-A888aLZc0cbu~xwfJFQCtqZ7C9~bN3tv2QBVaHduEFcwFefFNXyNLV4bXnZ8d31l24NVtwA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
BCR-ABL kinase activity in K562 and K562-R cells.
BCR-ABL was immunoprecipitated from cell lysates (400 μg) with anti-abl, washed extensively, resuspended in kinase buffer, and incubated with 500 nM STI571 or buffer alone (control) for 30 minutes. Kinase reactions were initiated by the addition of 10 μCi (0.37 MBq) [32P]-ATP and enolase. After 30 minutes at 25°C, reactions were quenched with the addition of 2 × sample buffer, and phosphoproteins were resolved by SDS-PAGE. Tyrosine phosphoproteins were detected by autoradiography. Autophosphorylation of BCR-ABL and phosphorylation of enolase are shown. Similar results were obtained using anti-bcr for immunoprecipitation (data not shown).
