Fig. 7.
![Fig. 7. Effect of CGP-76030 on tyrosine kinase activity of BCR-ABL and LYN in vitro. / BCR-ABL or LYN was immunoprecipitated from equal protein cell lysates (300 μg) from K562 (left) or K562-R (right) cells. Immune complexes were washed extensively, resuspended in kinase buffer or CGP-76030 (at the final concentration noted), and incubated for 30 minutes before kinase reactions were initiated with the addition of enolase and 10 μCi (0.37MBq) [32P]-ATP. Phosphoproteins were detected as described in the legend to Figure 3. (Top) Migration of phosphorylated BCR-ABL and enolase is depicted. (Bottom) Migration of phospho-LYN and enolase is shown. Note: LYN autophosphorylation is not detected in immune complexes from K562 cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/2/10.1182_blood.v101.2.690/4/h80233696007.jpeg?Expires=1736211480&Signature=Wr2qJt98bJ~HgiWBawU7D3X2JHiLwRqFQCAev0yAckt2Go8xSUUHpPJppuBlZiN1~RcTHcSRMgNqcAtUbgMoTMkbjS6YrUxtwL5PpNf8fkXBr6pI2oPPWsNRlBORP1AUqDUFk38iQLAi6dyqwCpk3AspFW9i4-86EKFNpbHZ6~db6RvZ6-vveC9PUPZO66F5hEV0xDiJaf2McP1KJF-cCBh~eBo04Um18LFj8vzMPRxK7HI~YAt1Yfu6spXR9i2Ye3YBxtIrU0U~8~RI1wDDc~aNnbESYQ0tenMZKXtOzF1cnDVdwf1nYhBWTvhYmPk5eZ6OPer9pd3GqQ6XZyWKcQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of CGP-76030 on tyrosine kinase activity of BCR-ABL and LYN in vitro.
BCR-ABL or LYN was immunoprecipitated from equal protein cell lysates (300 μg) from K562 (left) or K562-R (right) cells. Immune complexes were washed extensively, resuspended in kinase buffer or CGP-76030 (at the final concentration noted), and incubated for 30 minutes before kinase reactions were initiated with the addition of enolase and 10 μCi (0.37MBq) [32P]-ATP. Phosphoproteins were detected as described in the legend to Figure 3. (Top) Migration of phosphorylated BCR-ABL and enolase is depicted. (Bottom) Migration of phospho-LYN and enolase is shown. Note: LYN autophosphorylation is not detected in immune complexes from K562 cells.
