Fig. 3.
Fig. 3. Kinetics of HTLV Env receptor expression following TCR engagement. / Freshly isolated CD4+ lymphocytes were activated with immobilized αCD3 and αCD28 mAbs and expression of the HTLV Env receptor was followed after 2, 4, 8, 24, 72, and 216 hours of culture using HRBD. Cells were simultaneously evaluated for expression of the CD25 (IL-2Rα) and CD69 activation markers using PE-conjugated mAbs. Staining profiles are shown and filled histograms depict staining with an FITC-conjugated secondary mAb alone or with isotype-matched PE-conjugated control mAbs. Results are representative of data obtained in 4 independent experiments.

Kinetics of HTLV Env receptor expression following TCR engagement.

Freshly isolated CD4+ lymphocytes were activated with immobilized αCD3 and αCD28 mAbs and expression of the HTLV Env receptor was followed after 2, 4, 8, 24, 72, and 216 hours of culture using HRBD. Cells were simultaneously evaluated for expression of the CD25 (IL-2Rα) and CD69 activation markers using PE-conjugated mAbs. Staining profiles are shown and filled histograms depict staining with an FITC-conjugated secondary mAb alone or with isotype-matched PE-conjugated control mAbs. Results are representative of data obtained in 4 independent experiments.

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