Expression of the HTLV receptor on TCR-stimulated lymphocytes requires de novo protein synthesis.

Freshly isolated CD4⁺ lymphocytes were activated with immobilized αCD3 and αCD28 mAbs in the absence or presence of the protein synthesis inhibitor cycloheximide (CHX; 5 μg/mL) for 24 hours. The size and density of viable cells were monitored by forward angle light scatter (FSC) and side angle scatter (SSC) on a flow cytometer. HTLV receptor expression as well as CD25 and CD69 levels were assessed using H_RBD and the appropriate conjugated mAbs, in the absence and presence of CHX. Filled histograms depict control binding. Data are representative of results obtained in 2 independent experiments.