Mutation analysis of the CD11c gene promoter.

Contribution of Boxes A to H to CD11c promoter activity in (A) PMA-treated Mo hairy cells, (B) PMA-treated Jurkat T-lymphocytic cells, and (C) PMA-treated U937 monocytic cells. The portions of theCD11c gene used in transfection assays are illustrated on the left as filled bars, and the regions either deleted (Box A) or mutated (Boxes B to H) are represented by open boxes. The wild-typeCD11c promoter spanning nucleotides − 128 to + 36 is present in the luciferase expression construct p11Wt, whereas p11ΔA to ΔH represent the specific replacement of Boxes A to H, respectively, with the pATLuc polylinker sequence 5′-GCCAAGCT-3′. Expressed as hatched bars on the right are the levels ofluciferase gene activity corrected for transfection efficiency and after subtraction of the background activity conferred by the control plasmid pATLuc. The level of expression of p11Wt is assigned a value of 100%, and the expression level conferred by the deletion and mutation constructs is displayed as a proportion of this value. Each bar represents the mean ± the standard deviation of 3 independent transfection experiments.