Effect of U0126 on cell proliferation.

The hairy cell line EH and the promonocytic cell line U937 were either left untreated or equal numbers were treated in parallel with 8 μM U0126. After 24, 48, and 72 hours cells were counted and the number of untreated cells assigned a value of 100%. The number of treated cells is expressed as a percentage of this value. The bars depicting analysis of EH proliferation represent the mean ± SD of 6 independent experiments. The bars depicting analysis of U937 proliferation represent the mean ± SD of 5 independent experiments. The Student t test was used to derive probabilities of statistical significance. The P values for the drop in EH cell number after 24, 48, and 72 hours of treatment with U0126 were .015, .001, and .000, respectively. The P values for the change in U937 cell number after 24, 48, and 72 hours of treatment were .241, .089, and .203, respectively.