Fig. 7.
Synergistic activation of pleckstrin phosphorylation and phosphatidic acid production.
Platelets were labeled with 32P-orthophosphate for 1 hour prior to stimulation with submaximal concentrations of 0.1 μM U46619, 10 μM epinephrine (Epin), or both together (panel A) or 2 nM convulxin (Cvx), 10 μM epinephrine (Epin), or both together (panel B). Samples were harvested after 1 minute to determine the degree of pleckstrin phosphorylation (i) or phosphatidic acid production (ii), as described in “Materials and methods.” Results are the mean ± SEM of 3 independent experiments.
