SDS-PAGE analysis of recombinant protein C variants.

The purified protein C variants were subjected to 10% SDS-PAGE before and after activation by thrombin under both nonreducing (A) and reducing conditions (B). The proteins were visualized by silver staining. One microgram of protein was applied to each lane. Lanes 2 to 5 represent nonactivated protein C, whereas activated proteins were applied to lanes 6 to 9. Lanes 2 and 6 contain WT protein C; lanes 3 and 7, the QGN variant; lanes 4 and 8, the SEDY variant; and lanes 5 and 9, the QGNSEDY variant. Lane 1 contains the molecular weight markers. SC indicates single chain; HC, heavy chain; LC, light chain.