Anticoagulant activity of APC variants.

Increasing concentrations of the different APC variants were added to plasma-based clotting assays using (A) APTT or (B) tissue factor–containing reagent. QGNSEDY-APC, (▴); SEDY-APC, (▵); GNED-APC, (▿); QGN-APC, (■); WT APC, (●). Note the logarithmic y-axes. Each point is the mean of 3 independent experiments performed in duplicate, and the error bars represent the standard deviation.