Western blot analysis of FVa degradation.

Plasma-derived FVa (0.8 nM) was incubated with 25 μM PS/PC, 10:90, phospholipid vesicles at 37°C and 0.04 nM WT APC or QGNSEDY-APC. At the indicated times, aliquots were drawn and subjected to Western blot analysis using the monoclonal antibody AHV5146, which reacts with an epitope located between positions 306 and 506. HC, heavy chain of Fva; 506, the 1-506 fragment; 306, the 307-506 fragment.