Quantitative image capture/analysis of ISH signals in lymphoid tissues from uninfected and SIV-infected macaques.
Quantitative image analysis was used to determine the autoradiographic signal intensities following ISH for CCL19/MIP-3β, CCL21/6Ckine, CCR6, CCR7, DC-LAMP, DC-SIGN, and DECTIN-1 in lymph node (A, C, E) and spleen (B, D, F) in the absence of infection or during acute infection or AIDS. The percentage of surface area in individual microscopic fields that was covered by silver grains was determined, and the values for the background-subtracted ISH signals are shown. Statistical significance was examined by the Student t test.P < .05 has been noted with the following symbols: acute infection versus uninfected (*), AIDS versus uninfected (†), AIDS versus acute infection (#). Mean (geometric) plasma viral loads in these groups were: uninfected, less than 100 copies/mL; acute, 12.0 × 107 copies/mL; and AIDS, 1.8 × 106copies/mL.
