G-CSF–induced differentiation response of Cb2-EGFP– and EGFP-expressing 32D/G-CSF-R clones.

(A) There were 8 Cb2-EGFP–expressing clones and 4 EGFP control clones cultured for 9 days in the presence of G-CSF (see “Materials and methods”). The y-axis shows the percentage of neutrophils and the x-axis shows the days of culture. (B) Differential countings of 4 Cb2-EGFP–expressing clones and 4 EGFP control clones at day 9 of G-CSF culture. White represents blast cells, black represents intermediately matured granulocytic forms, and gray indicates terminally differentiated neutrophilic granulocytes.