Fig. 6.
G-CSF and IL-3 proliferative response of Cb2-EGFP– and EGFP-expressing 32D/G-CSF-R clones.
(A) Different proliferation curves of 8 Cb2-EGFP–expressing clones and 4 EGFP control clones stimulated with G-CSF. The y-axis indicates the number of cells × 105/mL. (B) [3H]-TdR incorporation of Cb2-EGFP–expressing cells or EGFP control cells at day 7 of culture in G-CSF (G) with or without the Cb2-specific antagonist (C2). The y-axis represents [3H]-TdR incorporation in counts per minute. (C) Proliferation curves of 8 Cb2-EGFP– and 4 EGFP-expressing clones stimulated with IL-3 during 9 days of culture. The y-axis indicates the number of cells × 105/mL.