Effect of signal transduction pathway inhibitors on the G-CSF differentiation response of 32D/G-CSF cells.

(A) Cb2-EGFP–expressing cells and EGFP control cells were cultured for 9 days in the presence of G-CSF plus U0126 (U) or PD98059 (PD). At the y-axis the percentage of neutrophils is shown, and at the x-axis the day of culture is indicated. (B) Morphologic analysis of a Cb2-EGFP–expressing clone and an EGFP control clone cultured in the presence of G-CSF plus PD98059, U0126, LY-294002, or dbcAMP. Pictures were taken at day 8 of culture except for incubations with U0126 (day 6). Original magnifications × 63.