HGAL mRNA expression by real-time RT-PCR.

(A) HGAL mRNA expression of normal tissues. (B) HGAL mRNA expression in cell lines. (C) HGAL mRNA expression in FCL (n = 21; ▪), DLBCL (n = 54; ▴), CLL (n = 16; ▾), MZL (n = 3; ⧫), MCL (n = 4; ●), T-LL (n = 2; □), and sorted and pooled GC cells from 3 normal tonsils (▵). HGAL mRNA expression was measured by real-time quantitative PCR. HGAL mRNA expression is a relative expression of HGAL normalized to GAPDH in relation to the HGAL/GAPDH ratio of the Raji cell line (see “Patients, materials, and methods”).